Diabetes disorder has acquired an epidemic level and a threat for world population. All parts of plant Sida cordifolia are utilized for hypoglycemic activity. Bioactive component of aqueous extract of Sida cordifolia were phenolic, flavonoids, non-tannin and tannin. To create diabetes in Wistar rat, streptozotocin solution (0.5%) was freshly prepared in 0.1 M sodium citrate buffer (pH 4.5) and administered (55mg/kg) in overnight fasted rats intraperitoneally. Rats of group-A served as control and diabetes was induced in rats of group-B, C, D and E. Diabetic rats of group-B designated as diabetic control were treated with carboxy methyl cellulose. Whereas diabetic rats of groups-C and D were treated with aqueous extract of Sida Cordifolia at two dosages (200mg/kg and 400mg/kg respectively) by oral route after mixing with carboxy methyl cellulose for 28 days. Rats of group-E were treated with Glibenclamide (5mg/kg) mixed in carboxy methyl cellulose for 28 days. Higher dose (400mg/kg) of Sida cordifolia significantly increases (P<0.05) body weight (186.66±6.67g) and significantly decreases blood glucose level (152.17±16.92mg/dl) of group-D diabetic rats on day 29th compared to day zero within the group. Also at same dose Sida cordifolia significantly (P<0.05) decreases total cholesterol (55.12±1.47mg/dl), triglycerides (45.95±1.56mg/dl), LDL (24.12±1.72mg/dl), plasma creatinine (0.54±0.07mg/dl), plasma urea nitrogen (58.59±3.25mmol/l), lipid peroxidation (5.90±0.34nmol MDA/ml) and significantly (P<0.05) increases HDL (34.76±1.66mg/dl), catalase (59.98±3.25Umol H2O2/min/mg of Hb) and superoxide dismutase (62.47±2.33U/mg of Hb) activity of group-D diabetic rats on day 29th compared to day zero within the group. The bioactive component of plant mainly flavonoids and alkaloids produces anti-diabetic activity.