DEVELOPMENT OF A LATEX AGGLUTINATION TEST FOR RAPID DIFFERENTIAL DIAGNOSIS OF VAGINAL INFECTIONS

International Journal of Innovations in Biological and Chemical Sciences, Volume 7, 2016, 8-10

Morteza Yousefi, Seyedeh Maryam Sharafi, Hossein Yousofi Darani

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ABSTRACT

Background: Vaginitis is a common problem among women. Appropriate laboratory diagnosis is important to succeed better treatment. Routine methods for laboratory diagnosis are not enough sensitive or time consuming. So construction of a latex agglutination kit for rapid diagnosis of infectious vaginitis has been investigated in this “study”.

Methods: Anti Trichomonas vaginalis and anti Candida albicans sera raised in rabbits. Antibodies were purified from the antisera using salt precipitation method. The antibodies were then conjugated to latex particles and used to detect the relevant antigens.

Results: When anti trichomonas coated latex particles mixed with Trichomonas vaginalis, agglutination was appeared.  Also agglutination was occurred when anti candida coated latex particles were mixed with Candida albicans antigen.

Conclusion: Latex agglutination test developed in this work could be a sensitive method for rapid differential diagnosis of infectious vaginitis.

Key words: latex agglutination, vaginal infection, laboratory diagnosis