Development of Gentamicin-loaded Solid lipid Nanoparticles: Evaluation of Drug Release Kinetic and Antibacterial activity against Staphylococcus aureus

International Journal of Pharmaceutical Research and Innovation, Volume 7, 2014, 1-6

Development of Gentamicin-loaded Solid lipid Nanoparticles: Evaluation of Drug Release Kinetic and Antibacterial activity against Staphylococcus aureus

Donya Kazemi, Mojtaba Salouti, Kobra Rostamizadeh, Arash Zabihian

 

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ABSTRACT

Context: The attention to solid lipid nanoparticles (SLNs) particularly in the field of drug delivery has increased during the recent years.

Objective: This research was designed to load gentamicin onto SLNs to produce a drug delivery system with controlled release.

Materials and methods: Ultrasonication/highspeed homogenization technique was carried out to prepare gentamicin-loaded SLNs. The tests including: size, zeta potential, drug release kinetics, stability and scanning electron microscopy were performed to evaluate in vitro characterization of the new nano-drug. Agar well diffusion and macrodilution methods were used to study the antibacterial activity of gentamicin-loaded SLNs against Staphylococcus aureus.

Results: The average particle size of gentamicin-loaded SLNs was 282.3 nm with zeta potential of +8.16 mV. The drug-loading efficiency was found 40%. The drug release study showed a gradual gentamicin release up to 96 h without significant burst effect.

Conclusion: Gentamicin-loaded SLNs is a good drug delivery system with desirable release characteristics.

Key words: Drug Delivery system; Solid lipid nanoparticles; Release Kinetic; Gentamicin; Staphylococcus aureus