Phytochemical Screening And In Vitro Antioxidant Activity Of Chloroform Extract Of Urena Sinuata (L.)

International Journal of Pharmaceutical Research and Innovation, Vol.6:19-24, 2013

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ABSTRACT:
The present study was designed to investigate antioxidant properties of chloroform extract of Urena sinuate along with phytochemical study for the presence of phytochemical constituents. Phytochemical analyses were found to be positive for carbohydrates and gum, reducing sugar, alkaloid, steroid, glycoside and flavonoids. Antioxidant potential was evaluated using DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging assays. In DPPH scavenging method, scavenging of DPPH was observed in different concentrations (20, 40, 60, 80, 100, 200, 400, 800μg/ml). Plant extract found to demonstrate significant scavenging activity which was found to increase with concentration of the extract with IC50 value of 10.64μg/ml while IC50 value of the reference ascorbic acid was 1.61μg/ml.
Keywords: Urena sinuate (L.), Phytochemical, DPPH scavenging method, Antioxidan