The present study optimized the regeneration protocol by using leaf explant in Vigna mungo (L) silvestris organogenesis. Primary immature leaf segment were inoculated on MS medium supplemented with 2, 4- dichlorophenoxyacetic acid (0.5 μM to 72.5 μM), naphthaleneacetic acid (0.5 μM to 72.0 µM). Callus initiation was observed in all media evaluated and the highest cell proliferation was obtained from explants cultivated in the presence of 2, 4-dichlorophenoxyacetic acid. Shoot induction was obtained from callus induced on 6.0 µM 2, 4-D at 6 weeks after transferring the callus and then regenerated plants were hardened and acclimated in greenhouse conditions. A medium supplemented with 6.0 µM 2, 4-D, 3.0% sucrose, was effective to achieve a high frequency of callus induction, maturation, and further development.