Abstract A study on the cultivation of Pleurotus ostreatus and P. pulmonarius mushroom on different substrates was conducted at Biological Sciences Department, Main Campus Ahmadu Bello University, Zaria. The aim was to investigate the influence of some substrates on the growth of Pleurotus ostreatus and P. pulmonarius so as to organically utilize these substrates and minimize the environmental nuisance caused by their burning. The highest yield was recorded from Gmelina arborea sawdust, followed by corn cobs. Gmelina arborea sawdust is considered the most suitable substrates for growing of Pleurotus ostreatus with no growth in P. pulmonarius. The chemical properties of the various substrates combined to influence the chemical endowment and growth of Pleurotus ostreatus.