Description

ABSTRACT

The purpose of this research was to isolate the novel biopolymer from the seeds of Juglans regia. The purpose of isolation from natural seeds was to evaluate the potentiality of biopolymer in delivery of nanosized lamotrigine as an antiepileptic drug. The biopolymer was isolated from the seeds of Juglans regia and characterized for its suitability in preparation of bionanoparticles in the form of bionanosuspension. The biopolymer was isolated by grinding the soaked seeds and then the juice was taken and centrifuged. The solution was treated with acetone and methanol separately and after refrigeration for 24 hours, the free flowing biopolymer was obtained after drying. Lamotrigine was nanosized by screening its nano size particle by UV method. The nanosized lamotrigine was used for preparation of bionanoparticles by sonication method. The isolated biopolymer confirms its polymeric nature in spectral analysis. The prepared bionanoparticles showed the release of lamotrigine in sustained manner over 36 hours. LJ7 showed up to 92.051% drug release in 36 hours. According to the release kinetic study the best fit model was found to be Koresmayer-peppas and the mechanism of drug release was found to be anomalous transport. Thus the obtained results of isolated biopolymer characterization from and other evaluation like % entrapment efficiency, particle size, release study, kinetic studies and stability study revealed that the isolated biopolymer has good potentiality  to form bionanoparticles and it can be safely used as an alternative to synthetic and semisynthetic polymers  for the preparation of lamotrigine loaded stable bionanoparticles.

Key words: Biopolymer, Bionanoparticles, Bionanosuspension, Lamotrigine, Epilepsy, Nanosizing Juglans regia seeds