Description

ABSTRACT

Fts-Z is the major cytoskeletal protein in the bacterial cytokinesis machine, and it is the first protein to move to the division site and is essential for recreating other proteins that produce new cell walls between dividing cells. The cellular concentration of Fts-Z regulates the frequency of division and genetic studies have indicated that it is the target of several endogenous division inhibitors. Antibiotic resistance has the potential to affect people at any stage of life. Since drug resistance too many antibiotics have been known hence it is essential to develop new drugs. Herbs can be antibiotics too, Tinospora cordifolia is a well-known medicinal plant used to cure plenty of diseases. The plant having anti-bacterial, anti-cancer, anti-diabetic, anti-inflammatory, antioxidant, anti-spasmodic, anti-pyretic, anti-allergic, anti-leprotic immunomodulatory activity, etc. Berberine is a natural plant alkaloid having many medicinal properties including anti-bacterial. In the present study, berberine was isolated by preparative HPLC and characterized by sophisticated instruments such as LC-MS, NMR, and IR. The isolated compound was tested on E. coli; berberine successfully inhibited the bacterial growth. Molecular docking was done to check the interaction of berberine with Fts-Z protein. Berberine docks well with Fts-Z protein and In silico analysis showed berberine as a good inhibitor of Fts-Z protein.

Keywords: Berberine, HPLC, LC-MS, Fts-Z, In silico