Description

ABSTRACT

To improve bioavailability, physical stability and more target specificity with predetermined drug release profile, it is necessary to prepare a unique dosage form like microspheres. In this research we prepared 0.25%, 0.50% and 0.75% w/v Diclofenac Gelatin microspheres using phase separation coacervation technique. The Diclofenac was estimated using UV-Visible spectroscopically at 276nm. The drug entrapment efficacy of 0.75% w/v Diclofenac Gelatin was found to be maximum i.e., 94.45 ± 0.7%. From the in-vitro drug release studies it was confirmed that 0.75% w/v Diclofenac Gelatin microspheres has reported 68.6 ± 1.4% drug entrapment efficacy; which indicates prolong release profiling of the formulation. From the particles size analysis, it was confirmed that all three batches were shown particles size in micro size range. From the TEM analysis it was confirmed that the prepared 0.75% w/v Diclofenac Gelatin microspheres were spherical with no aggregation and narrow distribution. From the motic microscopical images it was confirmed that the drug was properly encapsulated within the prepared microspheres. The outcome of the research indicates that, 0.75% Diclofenac Gelatin microspheres was physically more stable and capable of improving bioavailability of the Diclofenac sodium after oral administration.

Keywords: Diclofenac Gelatin microspheres, Transmission Electron Microscopy, Motic microscopy, Particle size, Drug entrapment efficacy, In-vitro drug release studies