ABSTRACT

Introduction: It is known that mitosis is a fundamental process and it has been researched in normal as well as in pathology. It is suggested that the cause of a colossal number of research about mitosis is an empirical approach to the problem. For example, nding the optimal combination (MF) of only nine components (cytokines or any proteins) requires 181440 steps.

Aim: In the study, the aging-related approach to detection of an explicit of mitotic factor MF to control mitosis through only one step is o ered.

Material and Methods: e following formula of MF has been invented: MF = ∆ (A–B). e formula includes: (a) Composition of the known components of thoracic duct lymph (TDL) of immature rabbits and (b) composition of the known components of mature rabbits TDL, ∆ – the di erence of the components composition between “A” and “B.” Results and Discussion: e approach should be carried out in the following succession: (1) To obtain samples of TDL of immature rabbits and TDL of mature rabbits (groups 1- “A” and 2- “B” accordingly) by special glass micropipette (Patent No 1495076, Russia), (2) to make lymph plasma from the samples of TDL of rabbits of group 1 and group 2, (3) to nd a qualitative and quantitative components composition of lymph plasma and TDL cells of group 1 and group 2, and (4) to nd the di erence (MF/∆) between the lymph plasma of the components composition of groups 1 and 2. Consequently, the di erence (∆) will be the sought-for MF. To study MF proteomic analysis can be used.

Conclusions: e aging-related approach can be used to study di erent features of TDL, particularly cells division, identi cation of lymph MF, that can play a key role in access to cell dividing mechanism, and it is the way to elaborate new cancer drugs.

Key words: Aging-related approach, mitosis, lymph